ارزیابی ترکیبات تام فنولی، فلاونوئیدی، آنتوسیانینی و فعالیت ضدباکتریایی و آنتی‌اکسیدانی عصاره استونی میوه ولیک

Background and Objective: Crataegus elbursensis is one of the important species of Rosaceae family and commonly found in the North of Iran. In this study, the antioxidant, antibacterial properties, total phenolic, flavonoid and anthocyanin contents in acetonic extract of Iranian Hawthorn (Crataegus elbursensis) fruits were examined. Materials and Methods: Extraction of fruit bioactive compound was done using %80 acetone. Total phenolic, flavonoid and anthocyanin contents were measured spectrophotometrically. The antioxidant capacity of the extract were assessed by DPPH (2,2-diphenyl-1- picrylhydrazyl) radical-scavenging activity and compared to synthetic antioxidant BHT (at different concentration of 50, 75, 100, 250, 500 μg/ml). In addition, the stability of the acetonic extract to pH (4, 7 and 9) and also antimicrobial activity of the extract by the broth microdilution method was investigated. Data were analyzed using Duncan's multiple test by SAS software Results: The extract showed higher radical scavenging activity than BHT. Also, the results of extract stability tests in different pH showed that antioxidant activity of the extract remained constant. The extract revealed significant antimicrobial activity against all of tested bacterial. The most efficient bactericidal activity of the extract was against Bacillus subtilis (MBC= 2.5 mg/ml) while Salmonella enteric was the most resistant bacterium (MBC= 20 mg/ml). Conclusion: The results obtained in this study clearly indicate that C. elbursensis studied proved to be a rich source of bioactive compounds with antioxidant and antimicrobial properties which could be used in food and drug industry and serve to protect human health. Key words: Antimicrobial activity, Total phenolic compounds, Antioxidant, Hawthorn fruit   Funding: This research was funded by Gorgan University of Agricultural Science & Natural Resources, Gorgan, Iran. Conflict of interest: None declared. Ethical approval: None declared.   How to cite this article: Salmanian SH, Sadeghi Mahoonak AR, Alami M, Ghorbani M Evaluation of Total Phenolic, Flavonoid, Anthocyanin Compounds, Antibacterial and Antioxidant Activity of Hawthorn (Crataegus Elbursensis) Fruit Acetonic Extract. J Rafsanjan Univ Med Sci 2014 13(1): 53-66. [Farsi]